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業績

原著論文

  1. Goto S, Kojima N, Komori M, Kawade N, Oshima K, Nadano D, Sasaki N, Horio F, Matsuda T, Miyata S. (2024) Vitamin C deficiency alters the transcriptome of the rat brain in a glucocorticoid-dependent manner, leading to microglial activation and reduced neurogenesis. J Nut Biochem. In Press. 10.1016/j.jnutbio.2024.109608.

  2. Suzuki H, Ito T,  Ogata T, Tsukahara Y, Nelson RS, Sasaki N, Matsushita Y. (2024) Overexpression of NtERF5, belonging to the ethylene response factor gene family, inhibits potato virus X infection and enhances expression of jasmonic acid/ethylene signaling marker genes in tobacco. J Gen Plant Pathol. published online. 10.1007/s10327-023-01166-w.

  3. Suzuki T, Fujita M, Suzuki M, Shigeta K, Nelson, RS, Matsushita Y, Sasaki N. (2024) Overexpression of BBF3 encoding a tobacco Dof transcription factor affects gene expression and cell death induction during N-mediated hypersensitivity to tobacco mosaic virus. J Gen Plant Pathol. 90: 2-13. 10.1007/s10327-023-01155-z.

  4. Yamato S, Sasaki N, Komatsu, K, Mashimo K, Motobayashi. (2023) Temporal changes in colonization levels of Ustilago esculenta in different parts of Zizania latifolia plants competent and incompetent for stem gall formation. J Gen Plant Pathol. DOI: 10.1007/s10327-023-01152-2

  5. Chigira Y, Sasaki N, Komatsu K, Mashimo K, Tanaka S, Numamoto M, Moriyama H, Motobayashi. (2023) Mating types of Ustilago esculenta infecting Zizania latifolia cultivars in Japan are biased toward MAT-2 and MAT-3. Microbes Environ. DOI: 10.1264/jsme2.ME23034

  6. Li D, Miyasaka Y, Kubota A, Kozono T, Kitano Y, Sasaki N, Fujii T, Tochio T, Kadota Y, Nishikawa A, Tonozuka T. (2023) Characterization and alteration of product specificity of Beijerinckia indica subsp. indica β-fructosyltransferase. Biosci Biotechnol Biochem. 6:zbad074. DOI: 10.1093/bbb/zbad074.

  7. Shinji H, Sasaki N, Hamim I, Itoh Y, Taku K, Hayashi Y, Minato N, Moriyama H, Arie T, Komatsu K. (2023) Dynamin-related protein 2 interacts with the membrane-associated methyltransferase domain of plantago asiatica mosaic virus replicase and promotes viral replication.
    Virus Res, 331:     199128. DOI: 10.1016/j.virusres.2023.199128

  8. Ding Y, Oyagi A, Miyasaka Y, Kozono T, Sasaki N, Kojima Y, Yoshida M, Matsumoto Y, Yasutake N, Nishikawa A, TonozukaT. (2022) Structural basis for proteolytic processing of Aspergillus sojae α-glucosidase L with strong transglucosylation activity. J Struct Biol, 214(3): 107874. DOI: 10.1016/j.jsb.2022.107874.

  9. Ogata T, Tsukahara Y, Ito T, Iimura M, Yamazaki K, Sasaki N, Matsushita Y. (2022) Cell death signaling is competitively but coordinately regulated by repressor-type and activator-type ethylene response factors in tobacco (Nicotiana tabacum) plants. Plant Biol, 24: 897-909. DOI: 10.1111/plb.13411

  10. Kubota A, Kawai R, Li D, Kozono T, Sasaki N, Nishikawa A, Fujii T, Tochio T, Tonozuka T. (2022) Enzymatic and structural characterization of β-fructofuranosidase from the honeybee gut bacterium Frischella perrara. Appl Microbiol Biotechnol. 106: 2455–2470．DOI: 10.1007/s00253-022-11863-9

  11. Shimizu N, Katagiri T, Matsumoto A, Matsuda Y,  Hiroshi Arai, Sasaki N, Abe K, Katase T, Ishida H, Kusumoto K, Takeuchi M, Yamagata Y. (2021) Oryzapsins, the orthologs of yeast yapsin in Aspergillus oryzae, affect ergosterol synthesis. Appl Microbiol Biotechnol 105(21-22):8481-8494. DOI: 10.1007/s00253-021-11639-7

  12. Novianti F, Sasaki N, Arie T, Komatsu K. (2021) Acibenzolar-S-methyl-mediated restriction of loading of plantago asiatica mosaic virus into vascular tissues of Nicotiana benthamiana. Virus Res. 306: 198585. DOI:10.1016/j.virusres.2021.198585.

  13. Komatsu K, Sasaki N, Yoshida T, Suzuki K, Masujima Y, Hashimoto M, Watanabe S,  Tochio N, Kigawa T, Yamaji Y, Oshima K, Namba S, Nelson R, and Arie T. (2021) Identification of a proline-kinked amphipathic α-helix downstream from the methyltransferase domain of a potexvirus replicase and its role in perinuclear complex formation and virus replication. J Virol. 90: e01906-20. DOI: 10.1128/JVI.01906-20

  14. Ikeda C, Taku K, Miyazaki T, Shirai R, Nelson R, Nyunoya H, Matsushita Y, Sasaki N. (2021) Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses. Sci Rep 11: 15424. DOI: 10.1038/s41598-021-94713-4

  15. Sasaki N, Murakami T, Yoshimoto N, Komatsu K, Matsushita Y, Nyunoya H. (2021) Cell death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata. J Gen Plant Pathol. 87: 170-177. DOI:10.1007/s10327-021-00984-0

  16. Koshiba R, Oba T, Fuwa A, Arai K, Sasaki N, Kitazawa G, Hattori M, Matsuda H, Yoshida T. (2021) Aggravation of food allergy by skin sensitization via systemic Th2 enhancement. Int Arch Allergy Immunol. 182: 292-300. DOI: 10.1159/000511239

  17. Sasaki N, Matsushita Y, Nyunoya H. (2019) Plant protein-mediated inhibition of virus cell-to-cell movement: far-western screening and biological analysis of a plant protein interacting with a viral movement protein. Methods Mol Biol. 2028:123‐144. DOI:10.1007/978-1-4939-9635-3_7

  18. Sasaki N, Takashima, E, Nyunoya H. (2018) Altered subcellular localization of a tobacco membrane raft-associated remorin protein by tobamovirus infection and transient expression of viral replication and movement proteins. Front Plant Sci 9:open access. DOI:10.3389/fpls.2018.00619

  19. Taku K, Sasaki N, Matsuzawa K, Okamura-Mukai A, Nyunoya H. (2018) Introns of the tobacco resistance gene N play important roles in elicitor-responsive upregulation and efficient induction of defense responses. J Gen Plant Pathol 84:73-84. DOI:10.1007/s10327-018-0762-y

  20. Li M, Yasuda M, Yamaya-Ito H, Maeda M, Sasaki N, Nagata M, Suzuki A, Okazaki S, Sekimoto H, Yamada T, Ohkama-Ohtsu N, Yokoyama T. (2017) Involvement of programmed cell death in suppression of the number of root nodules formed in soybean induced by Bradyrhizobium infection. Soil Sci Plant Nutr. 63:561-577. DOI:10.1080/00380768.2017.1403842

  21. Baba T, Hirose D, Sasaki N, Watanabe N, Kobayashi N, Kurashige Y, Karimi F, Ban T. (2016) Mycorrhizal Formation and Diversity of Endophytic Fungi in Hair Roots of Vaccinium oldhamii Miq. in Japan. Microbes Environ 31: 186-189. DOI:10.1264/jsme2.ME16011

  22. Sasaki N, Matsumaru M, Odaira S, Nakata A, Nakata K, Nakayama I, Yamaguchi K, Nyunoya H. (2015) Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense. Physiol Mol Plant Pathol 89: 70-77. DOI:10.1016/j.pmpp.2014.12.005

  23. Sasaki N, Shishikura R, Nyunoya H. (2014) Formation and intracellular movement of cytoplasmic bodies of the tomato mosaic virus 126-kDa replication protein in association with its movement protein. J Gen Plant Pathol 80: 272-281. DOI:10.1007/s10327-014-0515-5

  24. Takano M, Haque MA, Odaira S, Nakata K, Sasaki N, Nyunoya H. (2013) Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the tobacco mosaic virus resistance gene N and defense-related responses including ROS production. Plant Biotechnol. 30: 37-46. DOI:10.5511/plantbiotechnology.12.1207a

  25. Hiraguri A, Ueki S, Kondo H, Nomiyama K, Shimizu T, Ichiki-Uehara T, Omura T, Sasaki N, Nyunoya H, Sasaya T. (2013) Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus. J Gen Virol 94: 1145-1150. DOI:10.1099/vir.0.050005-0

  26. Sasaki N, Takaoka M, Sasaki S, Hirai K, Meshi T, Nyunoya H. (2013) The splice variant Ntr encoded by the tobacco resistance gene N has a role for negative regulation of antiviral defense responses. Physiol Mol Plant Pathol 84: 92-98. DOI:10.1016/j.pmpp.2013.08.002

  27. Hiraguri A, Hibino H, Hayashi T, Netsu O, Shimizu T, Uehara-Ichiki T, Omura T, Sasaki N, Nyunoya H, Sasaya T. (2012) The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles.  J Gen Virol 93: 2290-2298. DOI:10.1099/vir.0.044420-0

  28. Sasaki N, Odawara T, Deguchi M, Nyunoya H. (2012) Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris. Physiol Mol Plant Pathol 78: 38-44. DOI:10.1016/j.pmpp.2012.01.003

  29. Hiraguri A, Netsu O, Shimizu T, Uehara-Ichiki T, Omura T, Sasaki N, Nyunoya H, Sasaya T. (2011) The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus. Arch Virol 156: 911-916. DOI:10.1007/s00705-011-0939-6

  30. Sasaki N, Odawara T, Deguchi M, Matsushita Y, Nyunoya H. (2010) Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species. J Gen Plant Pathol 76: 69-73. DOI:10.1007/s10327-009-0214-9

  31. Harries P, Park J-W, Sasaki N, Ballard K, Maule A, Nelson R. (2010) Differing requirements for actin and myosin by plant viruses for sustained intercellular movement. Proc Natl Acad Sci USA 106: 17594-17599. DOI:10.1073/pnas.0909239106

  32. Gao J-S, Meng Y, Sasaki N, Kanegae H, Hayashi N, Nyunoya H. (2010) Characterization and cloning of TMV resistance gene homologues from Nicotiana tabacum. Afr J Biotechnol  9:7998-8006. DOI:10.5897/ajb10.732

  33. Sasaki N, Ogata T, Deguchi M, Nagai S, Tamai A, Meshi T, Kawakami S, Watanabe Y, Matsushita Y, Nyunoya H. (2009) Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement. Mol Plant Pathol 10: 161-173. DOI: 10.1111/j.1364-3703.2008.00517.x

  34. Haque MA, Sasaki N, Kanegae H, Mimori S, Gao J-S, Nyunoya H. (2009) Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. Physiol Mol Plant Pathol 73: 101-108. DOI:10.1016/j.pmpp.2009.03.001

  35. Gao J-S, Sasaki N, Kanegae H, Konagaya K, Takizawa K, Hayashi N, Okano Y, Kasahara M, Matsushita Y and Nyunoya H. (2007) The TIR-NBS but not LRR domains of two novel N-like proteins are functionally competent to induce the elicitor p50-dependent hypersensitive response. Physiol Mol Plant Pathol 71: 78-87. DOI:10.1016/j.pmpp.2007.11.002

  36. Sasaki N, Park J-W, Maule A, Nelson R. (2006) The cysteine-histidine rich region of the movement protein of Cucumber mosaic virus contributes to plasmodesmal targeting, zinc binding and pathogenesis. Virology 349: 396-408. DOI:10.1016/j.virol.2006.02.035

  37. Takeda A, Nakamura W, Sasaki N, Goto K, Kaido M, Okuno T, Mise K. (2005) Natural isolates of Brome mosaic virus with the ability to move from cell to cell independently of coat protein. J Gen Virol 86:1201-11.DOI:10.1099/vir.0.80775-0

  38. Sasaki N, Kaido M, Okuno T, Mise K. (2005) Coat protein-independent cell-to-cell movement of bromoviruses expressing brome mosaic virus movement protein with an adaptation-related amino acid change in the central region. Arch Virol 150:1231-1240. DOI:10.1007/s00705-004-0481-x

  39. Sasaki N, Kaido M, Okuno T, Mise K. (2004) Effects of artificial codon changes in the movement protein gene on adaptation of a hybrid bromovirus to cowpea. Microbiol Immunol. 48:131-135. DOI:10.1111/j.1348-0421.2004.tb03499.x

  40. Sasaki N, Arimoto M, Nagano H, Mori M, Kaido M, Mise K, Okuno T. (2003) The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses. Arch Virol 148:803-812. DOI:10.1007/s00705-002-0952-x

  41. Sasaki N, Fujita Y, Mise K, Furusawa I. (2001) Site-specific single amino acid changes to Lys or Arg in the central region of the movement protein of a hybrid bromovirus are required for adaptation to a nonhost. Virology 279:47-57. DOI:10.1006/viro.2000.0518


解説・総説

  1. 佐々木信光，多久和夫，丹生谷博：ウイルス防御応答における抵抗性遺伝子Nのイントロンの役割．（2018）植物感染生理談話会論文集（第53号）. p27-36.

  2. Hiraguri A, Netsu O, Sasaki N, Nyunoya H, Sasaya T. (2014)  Recent progress in research on cell-to-cell movement of rice viruses. Front Microbiol 20:open access https://doi.org/10.3389/fmicb.2014.00210

  3. Sasaki N. (2012) Studies on the molecular mechanism of cell-to-cell movement of plant RNA viruses. J Gen Plant Pathol 78: 423-424. DOI:10.1007/s10327-012-0406-6

  4. 三瀬和之, 藤崎恒喜, 佐々木信光, 竹田篤史, 岩橋福松, 辻修平, 海道真典, 奧野哲郎：ブロモウイルス感染における宿主特異性決定機構．(2005) 植物感染生理談話会論文集（第41号）. p101-110.


外部資金の獲得
・科学研究費補助金（代表）

  1. 令和元年度〜3年度　基盤研究C 「ウイルス抵抗性遺伝子Nのエリシター応答性におけるイントロンの未知機能の解明」　

  2. 平成29年度〜30年度　　挑戦的研究（萌芽）「植物免疫応答解析における葉面熱流変動測定法の確立と応用」　

  3. 平成26年度〜28年度　　基盤研究C 「細胞死に依存しない植物抵抗性誘導系を活用したウイルス細胞間移行の抑止機構の研究」

  4. 平成24年度〜25年度　　若手研究B 「転写仲介因子を介した植物ウイルスの細胞間移行制御機構の研究」　

  5. 平成20年度〜21年度　　若手研究B 　｢ウイルス移行タンパク質と相互作用する植物因子KELPによる感染抑制作用の研究｣

  6. 平成18年度〜19年度　　若手スタートアップ　｢植物ウイルス移行タンパク質と相互作用する植物KELPタンパク質の機能解析｣


・科学研究費補助金（分担）

  1. ​​令和5年度〜7年度　基盤研究C　「ポテックスウイルスの複製複合 体形成メカニズムの解明」

  2. 平成22年度〜24年度　　基盤研究C　｢タバコモザイクウイルス抵抗性遺伝子の転写調節と過敏感応答誘導機構の解明｣


・その他外部資金

  1. 令和5年度〜9年度　地球規模課題対応国際科学技術協力プログラム（SATREPS）「バナナ萎凋病の診断・警戒システムと発病制御戦略の構築と実装」

  2. 令和元年度〜3年度　GIR戦略的研究チーム採択「寄生性微生物による宿主操作の分子メカニズムの解明」（代表）

  3. 平成27年度　学長裁量経費海外渡航支援　「ペルー原産ナス科有用遺伝資源植物の探索および遺伝子解析」（代表）

  4. 平成26年度 一般財団法人 東京農工大学教育研究振興財団教育研究援助事業　「イントロンを介した植物病害抵抗性遺伝子の発現調節機構の研究」（代表）

  5. 平成24〜27年度 文部科学省特別経費「大学固有の生物資源を用いた放射性元素除去技術，バイオ肥料・植物保護技術」（分担）

  6. 平成23年度　財団法人　新技術振興渡辺記念会　科学技術調査研究助成　 ｢遺伝子組換えウイルス病害抵抗性植物開発に関する研究の動向調査｣　（代表）

  7. 平成22年度 連携リングによる研究院を越えた融合，萌芽的研究に対する支援経費　「ウイルス強耐性植物の作出に向けた病害抵抗性誘導機構の研究」（代表）

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アンカー 1
アンカー 2
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東京農工大学

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応用生物科学科（学部）

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応用生命化学コース（修士課程）

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連合農学研究科（博士課程）

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グローバルイノベーション研究院

遺伝子実験施設

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